Acquired Electronics360

Medical Devices and Healthcare IT

Real-Time Data Acquisition with Super-Resolution Microscopy Camera

11 July 2017

Image credit: Andor TechnologyImage credit: Andor Technology

A new super-resolution microscopy technology is now available on single photon sensitive iXon EMCCD cameras from Andor Technology Ltd. (Andor), Belfast, Northern Ireland, an Oxford Instruments company. ‘SRRF-Stream’ unlocks real-time super-resolution fluorescence microscopy on most modern microscopes, using conventional fluorophores at low illumination intensities, thus making it highly compatible with live cell imaging. A resolution improvement from 2- to 6-fold (50-150 nm final resolution) can be expected for most datasets.

The company adopted the SRRF technology recently developed at the University College London and enhanced it to run optimally on iXon EMCCD cameras. Advanced GPU processing optimization techniques were applied to execute the SRRF algorithm up to 30 times faster than the existing Image-based post processing implementation of SRRF. This significant acceleration enables workflow enhancement by allowing data acquisition and SRRF processing to operate in parallel.

Since processing is faster than the camera can acquire data, SRRF-Stream now accomplishes real-time super-resolution, with large field of view super-resolution images. An EMCCD full field of view super-resolved image can be readily generated at a rate of > 1 Hz, easily pushing up to > 10 Hz through use of smaller ROI sizes. Regarding image acquisition software, SRRF-Stream is readily available to SDK users and has also been made compatible with the MicroManager (Open Imaging) open source microscopy software platform.



Powered by CR4, the Engineering Community

Discussion – 0 comments

By posting a comment you confirm that you have read and accept our Posting Rules and Terms of Use.
Engineering Newsletter Signup
Get the Engineering360
Stay up to date on:
Features the top stories, latest news, charts, insights and more on the end-to-end electronics value chain.
Advertisement
Weekly Newsletter
Get news, research, and analysis
on the Electronics industry in your
inbox every week - for FREE
Sign up for our FREE eNewsletter
Advertisement

CALENDAR OF EVENTS

Date Event Location
12-16 Aug 2018 Vancouver, Canada
11-13 Sep 2018 Novi, Michigan
27 Sep 2018 The Reef, Los Angeles
03-05 Oct 2018 Boston, Massachusetts
26 Oct 2018 Old Billingsgate
Find Free Electronics Datasheets
Advertisement