A new super-resolution microscopy technology is now available on single photon sensitive iXon EMCCD cameras from Andor Technology Ltd. (Andor), Belfast, Northern Ireland, an Oxford Instruments company. ‘SRRF-Stream’ unlocks real-time super-resolution fluorescence microscopy on most modern microscopes, using conventional fluorophores at low illumination intensities, thus making it highly compatible with live cell imaging. A resolution improvement from 2- to 6-fold (50-150 nm final resolution) can be expected for most datasets.
The company adopted the SRRF technology recently developed at the University College London and enhanced it to run optimally on iXon EMCCD cameras. Advanced GPU processing optimization techniques were applied to execute the SRRF algorithm up to 30 times faster than the existing Image-based post processing implementation of SRRF. This significant acceleration enables workflow enhancement by allowing data acquisition and SRRF processing to operate in parallel.
Since processing is faster than the camera can acquire data, SRRF-Stream now accomplishes real-time super-resolution, with large field of view super-resolution images. An EMCCD full field of view super-resolved image can be readily generated at a rate of > 1 Hz, easily pushing up to > 10 Hz through use of smaller ROI sizes. Regarding image acquisition software, SRRF-Stream is readily available to SDK users and has also been made compatible with the MicroManager (Open Imaging) open source microscopy software platform.